Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

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4.4K Views

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07:54 min

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March 31st, 2021

DOI :

10.3791/62295-v

March 31st, 2021

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Raphael Nyaruaba¹^,²^,³, Xiaohong Li¹, Caroline Mwaliko²^,³^,⁴, Changchang Li¹, Matilu Mwau⁵, Nelson Odiwour¹^,²^,³, Elishiba Muturi¹^,²^,³, Caroline Muema¹^,²^,³, Junhua Li¹, Junping Yu¹, Hongping Wei¹

¹Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, ²International College, University of Chinese Academy of Sciences, ³Sino-Africa Joint Research Center, ⁴CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, ⁵Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

Podsumowanie

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SARS CoV 2 Detection

Multiplex DdPCR Assays

Two step Reverse Transcription

Nucleic Acid Quantification

Thermal Cycler

Automated Droplet Generation

96 well Plate

PCR Plate Sealer

Rozdziały w tym wideo

0:04

Introduction

0:48

cDNA and Droplet Digital Polymerase Chain Reaction (ddPCR) Preparation

2:03

Automated Droplet Generation

2:59

PCR Amplification and Droplet Reading

4:09

Data Analysis

6:01

Results: SARS‐CoV‐2 Detection Using a Two‐Color ddPCR System

7:07

Conclusion

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