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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

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These protocols can be used to develop in-house multiplex ddPCR assays for detection of SARS-CoV-2 using a two-color ddPCR system. This is useful for people who want to develop their own kits for the detection of SARS-CoV-2 and other pathogens. Co

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This work summarizes steps on developing different assays for SARS-CoV-2 detection using a two color ddPCR system. The steps are elaborate and notes have been included on how to improve the assays and experiment performance. These assays may be used for multiple SARS-CoV-2 RT-ddPCR applications.

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