Methods Article
Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
3.2K Views
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09:34 min
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February 17th, 2022
DOI :
February 17th, 2022
•Rozdziały w tym wideo
0:04
Introduction
1:02
Ex Vivo T Cell Isolation from Peripheral Blood Mononuclear Cells (PBMCs)
2:47
T Cell Stimulation and T Cell Expansion
3:54
Transduction of Lentiviruses on Stimulated T Cells
5:10
Surface Staining of B Cell Maturation Antigen (BCMA)-CAR Using Goat Anti-Mouse IgG (H+L) and Intracellular Staining of NIS Using Anti-Human ETNL NIS
7:18
NIS+BCMA-CAR T Cell In Vivo Imaging Using BCMA+OPM-2 Xenograft Mouse Model
7:57
Results: T Cell Growth Kinetics and In Vivo Cell Trafficking Assay
9:08
Conclusion
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