JoVE Journal

Cancer Research

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Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles

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2.0K Views

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09:57 min

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June 28th, 2021

DOI :

10.3791/62683-v

June 28th, 2021

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M. M. Hasibuzzaman¹^,², Kathleen A. Ross³^,⁴, Aliasger K. Salem¹^,⁴^,⁵^,⁶, Balaji Narasimhan³^,⁴, Andrean L. Simons¹^,²^,³^,⁵^,⁶^,⁷

¹Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, ²Department of Pathology, University of Iowa, ³Department of Chemical and Biological Engineering, College of Engineering, Iowa State University, ⁴Nanovaccine Institute, Iowa State University, ⁵Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, ⁶Holden Comprehensive Cancer Center, University of Iowa, ⁷Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa

Podsumowanie

A standard protocol is described to study the antitumor activity and associated toxicity of IL-1α in a syngeneic mouse model of HNSCC.

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In Vivo Antitumor Activity

Polyanhydride Nanoparticles

Immune Modulators

Immune Cell Populations

Tumor Implantation

Anti cancer Research

Toxicity Studies

Murine EERL Cells

Injection Technique

Blood Collection

Subcutaneous Injection

Rozdziały w tym wideo

0:04

Introduction

0:55

Preparation and Maintenance of HNSCC Cell Line

2:38

Tumor Implantation

3:14

Blood Collection and Serum Separation

4:20

Multiplexing of Collected Serum

4:51

Collection of Tumor and Inguinal Lymph Node and Preparation of Single-Cell Suspension

5:58

FACS Staining of Single-Cell Suspension

7:42

Results: IL-1α Antitumor Activity in HNSCC Mouse Model

9:03

Conclusion

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