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JoVE Journal
Cancer Research
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Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
1.7K Views
•
09:37 min
•
August 25th, 2021
DOI :
August 25th, 2021
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Rozdziały w tym wideo
0:05
Introduction
0:52
Tumor Dissection and Staining
1:46
Ex Vivo Ablation of Floxed Erbb4 Alleles in MPNST Cells
3:52
Proliferation Assays in MPNST Cells with Ablated Erbb4 Alleles
4:23
RNA-Seq Analyses and Gene Identification with Altered Expression by Erbb4 Loss
6:04
Orthotopic Allograft of MPNST Cells with Ablated Erbb4 Alleles and Erbb4 Ablation Effects Analysis
7:10
Results: Effect of Adenovirus Transduction on MPNST Cells and Allografts
9:09
Conclusion
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