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Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

1.7K Views

09:37 min

August 25th, 2021

DOI :

10.3791/62740-v

August 25th, 2021


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Gene Functions

Rozdziały w tym wideo

0:05

Introduction

0:52

Tumor Dissection and Staining

1:46

Ex Vivo Ablation of Floxed Erbb4 Alleles in MPNST Cells

3:52

Proliferation Assays in MPNST Cells with Ablated Erbb4 Alleles

4:23

RNA-Seq Analyses and Gene Identification with Altered Expression by Erbb4 Loss

6:04

Orthotopic Allograft of MPNST Cells with Ablated Erbb4 Alleles and Erbb4 Ablation Effects Analysis

7:10

Results: Effect of Adenovirus Transduction on MPNST Cells and Allografts

9:09

Conclusion

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