Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
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2.2K Views
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06:48 min
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April 29th, 2022
DOI :
April 29th, 2022
•Katsunori Yanai1, Shohei Kaneko1, Hiroki Ishii1, Akinori Aomatsu1,2, Kenichi Ishibashi3, Yoshiyuki Morishita1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University
Rozdziały w tym wideo
0:04
Introduction
0:39
Serum Sample Collection
1:37
Total RNA Extraction from Serum and cDNA Synthesis
3:33
qRT-PCR of miRNA
5:27
Results: Differentially Expressed miRNAs in the Kidneys and Serum of SAMP1 Mice
6:15
Conclusion
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