Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

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4.1K Views

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07:48 min

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November 10th, 2021

DOI :

10.3791/63332-v

November 10th, 2021

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Yashar Bashirzadeh*¹, Nadab Wubshet*¹, Thomas Litschel², Petra Schwille³, Allen P. Liu¹^,⁴^,⁵^,⁶

¹Department of Mechanical Engineering, University of Michigan, Ann Arbor, ²John A. Paulson School of Engineering and Applied Sciences, Harvard University, ³Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, ⁴Department of Biomedical Engineering, University of Michigan, Ann Arbor, ⁵Department of Biophysics, University of Michigan, Ann Arbor, ⁶Cellular and Molecular Biology Program, University of Michigan, Ann Arbor

Podsumowanie

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Rapid Encapsulation

Reconstituted Cytoskeleton

Actin Binding Proteins

Oil lipid Mixture

3D printed Encapsulation Chamber

Lipid oil Dispersion

Molecular Pathways

Rozdziały w tym wideo

0:05

Introduction

0:48

Preparation of Oil-Lipid-Mixture

2:12

Vesicle Generation

5:42

Imaging and 3D image Reconstruction

6:53

Results: Encapsulation of Actin Bundle Structures

7:08

Conclusion

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