Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

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6.8K Views

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07:33 min

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April 30th, 2019

DOI :

10.3791/59555-v

April 30th, 2019

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Masamune Morita¹, Yuri Ota¹^,², Kaoru Katoh¹, Naohiro Noda¹^,²

¹Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), ²Department of Life Science and Medical Bioscience, Waseda University

Podsumowanie

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Bacterial Cell Culture

Optical Density OD 600

Sucrose Solution

Giant Vesicle Formation

Aqueous Solution

Droplet Formation

Rozdziały w tym wideo

0:04

Title

0:31

Lipid-containing Oil Solution and Small Vesicle (SV) Preparation

1:54

Bacterial Cell Pre-culture

2:45

Giant Vesicle (GV) and Water-in-Oil (W/O) Bacterial Cell-containing Droplet Formation

4:11

Handmade Chamber and Supported Bilayer Membrane Preparation

4:54

GV Immobilization and Microscopic Bacterial Growth Observation

6:04

Results: Representative GV Containing Bacteria and Bacterial Growth Evaluation

6:44

Conclusion

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