HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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10:10 min
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March 31st, 2019
DOI :
March 31st, 2019
•Rozdziały w tym wideo
0:04
Title
0:49
The High Titer sgRNA Library Lentivirus Generation
1:44
Titration of Lentiviral Library in MOLM13 Leukemia Cells
2:59
Transduction of the Pooled CRISPR-Cas9 KO Library
4:20
Screening of the Pooled CRISPR-Cas9 KO Library with One-Step RT-qPCR
5:41
Verification of Integrated sgRNAs Positive Clones through Genotyping and Sanger Sequence
6:37
Detection of sgRNAs Induced Indel Mutation by Nuclease Digestion Assay
7:48
Results: Pooled CRISPR-Cas9 KO Library Screening and Identification
9:19
Conclusion
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