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Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

2.1K Views

12:30 min

March 16th, 2022

DOI :

10.3791/63569-v

March 16th, 2022


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Confocal Microscopy

Rozdziały w tym wideo

0:04

Introduction

1:07

Bovine Chromaffin Cell Culture

2:52

Transfection with Electroporation

4:18

Preparation for Patch-Clamp Recording and Confocal Imaging

5:41

Patch-Clamp Recording and Confocal Imaging

9:01

Confocal Imaging Data Analysis

10:21

Results: Whole-Cell Voltage-Clamp Recordings and Visualization of Fusion Events Under Confocal Microscope

11:52

Conclusion

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