A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

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2.7K Views

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07:20 min

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May 10th, 2022

DOI :

10.3791/63941-v

May 10th, 2022

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Kenta Yamauchi¹^,², Shinichiro Okamoto¹^,²^,³, Megumu Takahashi¹^,²^,⁴^,⁵, Masato Koike²^,³, Takahiro Furuta⁶, Hiroyuki Hioki¹^,²^,⁷

¹Department of Neuroanatomy, Juntendo University Graduate School of Medicine, ²Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, ³Advanced Research Institute for Health Sciences, Juntendo University, ⁴Department of Neuroscience, Graduate School of Medicine, Kyoto University, ⁵Japan Society for the Promotion of Science, ⁶Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, ⁷Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine

Podsumowanie

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Tissue Clearing Method

Permeabilized Brain Slices

Incubation Process

ScaleS0 And ScaleS4 Solutions

3D Printed Chamber Frame

Imaging Chamber Preparation

Agarose Solution

Microscopy Techniques

Rozdziały w tym wideo

0:04

Introduction

0:57

Tissue Clarification

2:06

Brain Slice Mounting

3:52

Confocal Laser Scanning Microscopy Imaging of Cleared Tissues

5:38

Results: Neuronal Imaging in Mouse Brain Slice Cleared with Sca/eSF

6:42

Conclusion

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