In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

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1.9K Views

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08:20 min

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August 17th, 2022

DOI :

10.3791/63987-v

August 17th, 2022

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Luna de Lacerda*¹, Guilherme Castro*¹^,², Cristopher Gomes¹, Caroline Junqueira¹

¹Instituto René Rachou, Fundação Oswaldo Cruz, ²Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais

Podsumowanie

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In Vitro Assay

Plasmodium

Red Blood Cells

Cytotoxic Lymphocytes

Effector Cell Population

Centrifugation

Rozdziały w tym wideo

0:04

Introduction

0:58

Experimental Malaria Infection in Mouse

1:49

Obtaining Fresh Whole Mouse Splenocytes

3:30

Cytotoxic Effector Cell Purification: CD8a⁺ T Cell Negative Selection

4:22

P. yoelii Infected RBC Isolation

5:21

CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture

6:22

Results: P. yoelii - iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii - iRBCs by CD8⁺ lymphocytes

7:39

Conclusion

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