Generating Genetically Modified Plasmodium berghei Sporozoites

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1.1K Views

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10:16 min

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May 5th, 2023

DOI :

10.3791/64992-v

May 5th, 2023

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Carson Bowers¹, Samarchith P. Kurup¹^,²

¹Center for Tropical and Emerging Global Diseases, University of Georgia, ²Department of Cellular Biology, University of Georgia

Podsumowanie

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Gradient Centrifugation

Giemsa Stain

Blood Smear Preparation

Isolation Of Schizonts

Rozdziały w tym wideo

0:04

Introduction

0:57

Generation of Schizonts in Culture

2:25

Transfection of Plasmodium Schizonts

4:58

Selection of the Transfected Parasites

5:52

Infection of Mosquitoes with the Transgenic Lines

7:15

Collection of Sporozoites

8:53

Results: Detecting the Lysis of Plasmodium and its Parasitophorous Vacuole in Host Hepatocytes

9:42

Conclusion

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