Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
4:22
P. yoelii Infected RBC Isolation
5:21
CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
6:22
Results: P. yoelii - iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii - iRBCs by CD8+ lymphocytes
7:39
Conclusion
필기록
This assay methodology, it's a major advancement in the knowledge of cell immunity against blood stage malaria, helping to uncover new therapeutic targets and to accelerate the progress of malaria vaccine. The establishment of a plasmodium in vitr
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Here we describe a new method to help elucidate the mechanisms of cellular immunity to Plasmodium during the blood stage of infection. This is an in vitro assay that measures infected red blood cell killing by cytotoxic lymphocytes.