JoVE Journal

Cancer Research

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Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

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4.6K Views

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12:41 min

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December 23rd, 2022

DOI :

10.3791/64434-v

December 23rd, 2022

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Maxim Le Compte¹, Edgar Cardenas De La Hoz², Sofía Peeters¹, Evelien Smits¹, Filip Lardon¹, Geert Roeyen¹^,³, Steve Vanlanduit², Hans Prenen¹^,⁴, Marc Peeters¹^,⁴, Abraham Lin¹^,⁵, Christophe Deben¹

¹Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, ²Industrial Vision Lab, University of Antwerp, ³Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), ⁴Department of Oncology, University Hospital Antwerp (UZA), ⁵Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp

Podsumowanie

This protocol describes a semi-automated method for medium- to high-throughput organoid drug screenings and microscope-agnostic, automated image analysis software to quantify and visualize multiparametric, single-organoid drug responses to capture intratumor heterogeneity.

Przeglądaj więcej filmów

Multiparametric Tumor Organoid

Widefield Live Cell Imaging

Single Organoid Analysis

Ex Vivo Organoid Screening

Patient derived Tumor Organoids PDTOs

Extracellular Matrix ECM

Pancreatic Ductal Adenocarcinoma PDAC

Cell Suspension

Dissociation Enzyme

Experimental Protocol

Clinical Therapy Response

Automated Analysis Pipeline

Rozdziały w tym wideo

0:05

Introduction

0:53

Day 0: Preparation of the 2- or 3-Day-Old Organoids

2:55

Days 2 - 3: Harvesting the 2- or 3-Day-Old Organoids

4:16

Seeding the 2- or 3-Day-Old Organoids Using a Pipetting Robot

5:27

Day 4: Drug Treatment and Reagent Dispensing with Digital Drug Dispenser

7:49

Image Acquisition with the Live-Cell Imager and Analysis of Data

10:12

Results: The Single-Organoid Dose-Response Analysis

12:03

Conclusion

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