Unique Approach for Isolating Rat Bone Marrow Neutrophils with Comparable Capacity

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1.7K Views

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08:56 min

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April 26th, 2024

DOI :

10.3791/65506-v

April 26th, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang²^,³, Zhenghua Xiao¹^,⁴, Li He¹^,⁴, Chaoyi Qin¹^,⁴

¹Department of Cardiovascular Surgery, West China Hospital, Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital, Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital, Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University

Podsumowanie

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Rat Neutrophils

Bone Marrow Isolation

Neutrophil Extracellular Traps

NETs Extraction

Isolation Techniques

Peripheral Blood Comparison

Purification Methods

Cellular Components

Inflammation Research

Autoimmune Diseases

Experimental Optimization

Viable Neutrophils

Rozdziały w tym wideo

0:00

Introduction

1:24

One and Two‐Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)

4:20

Acquisition and Verification of Rat Neutrophil Extracellular Traps (NETs)

6:52

Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs)

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