Microbiological Rapid On-Site Evaluation for Pulmonary Infectious Diseases

Our intent to introduce a real time rapid assessment method, M-ROSE, to assist physicians for formulating accurate treatment strategies for patient with primary infections. Rapid cell staining technology and a high definition microscopic imaging system are currently used to advance research in the field. The number of clinical studies on M-ROSE is relatively limited.

The biggest challenge is interpretation of M-ROSE results, which requires personnel, and well trained personnel as it is difficult to visually identify pathogens and differentiate between infection and contamination. With a better understanding of pathogens cause infections in real time, physicians can avoid an unnecessary broad spectrum antibiotics contributing to antibiotic resistance and to enhance the treatment of primary infections. M-ROSE is real time and highly efficient.

M-ROSE enables the macroscopic identification of pathogens such as aspergillus, cryptococcus, pneumocystis and candida. It holds significant guiding implications in sizing respiratory basic quality, distinguishing infectious from non-infectious disease, discriminating infection from contamination, as well as evaluating infection, civil royalty and prognose. To begin, obtain all the required materials for M-ROSE and place the complete set of Diff-Quik or DQ staining solutions in glass staining jars with sealed lids.

For roll slide preparation, extract tissue particles with a 2.5 to five milliliter disposable syringe needle. Then spread a circular area of moderate thickness from the inner to the outer one third section of the cytology slide. Submerge the slide in diff a solution for 10 to 30 seconds.

To remove excess diff a solution, put the slide in the PBS dye bath and rock it slightly up and down. Then gently shake off any remaining buffer. Immerse the slide in diff b solution for 20 to 40 seconds.

Then put the slide into the water dying tank and rock it slightly up and down. Finally, using absorbent paper, wipe off the residual liquid from the slide and proceed for microscopic examination. M-ROSE of patient bronchoscopic biopsy sample using DQ staining identified pneumocystis characterized by a clear structure, sharp contrast, and ascus.