P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

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10.6K Views

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10:26 min

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June 15th, 2020

DOI :

10.3791/61069-v

June 15th, 2020

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Carlos Victor Montefusco-Pereira*¹^,², Justus C. Horstmann*¹^,², Thomas Ebensen³, Christoph Beisswenger⁴, Robert Bals⁴, Carlos A. Guzmán³, Nicole Schneider-Daum¹, Cristiane de Souza Carvalho-Wodarz¹, Claus-Michael Lehr¹^,²

¹Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), ²Department of Pharmacy, Saarland University, ³Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, ⁴Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital

Podsumowanie

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P Aeruginosa

3D Co culture

Bronchial Epithelial Cells

Macrophages

Air liquid Interface

Preclinical Evaluation

Aerosol Administration

Human Cells Model

CFBE41 O Minus Cells

Cell Medium Sterility

Trypsin EDTA

Automated Cell Counter

Rozdziały w tym wideo

0:04

Introduction

1:26

Growth and Differentiation of Cells in Permeable Support Inserts

5:29

Infection by P. aeruginosa

7:00

Measurement of Bacterial Proliferation via Colony-forming Units (CFU)

8:00

Results: Epithelial-macrophage Co-cultures Infected with P. aeruginosa

9:48

Conclusion

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