Due to the challenges associated with conducting clinical trials on pouchitis, animal models can serve as valuable tools for studying pouchitis drugs and their mechanisms. This protocol describes the method for establishing a rat model of pouchitis. This experimental animal model of pouchitis provides a foundation for studying the pathogenesis and the treatment of the condition.
With previous experience in mouse feeding and IPAA surgery modeling, compressing the learning curve down to around 10 subjects is feasible. A critical step in this process is construction and anastomosis of the J-pouch. Postoperative management also play a pivotal role in successful modeling.
The successful establishment of the rat pouchitis model provide a solid platform for subsequent drug and mechanism research. Our team will always be committed to studying the pathogenesis of pouchitis and the mechanism of therapeutic drugs.
