<meta charset="utf8"></head><body>潰瘍性大腸炎の病因と治療を研究するための嚢炎の実験的ラットモデル

<ol>
	<li>Yang, M. L., Brar, M. S., Kennedy, E. D., De Buck Van Overstraeten, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+versus+transanal+IPAA+for+ulcerative+colitis:+A+patient-centered+treatment+trade-off+study.">Laparoscopic versus transanal IPAA for ulcerative colitis: A patient-centered treatment trade-off study.</a> Dis Colon Rectum. 67 (1), 107-113 (2024).</li><li>Aktas, M. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+status+and+surgical+technique+for+restorative+proctocolectomy+with+ileal+pouch-anal+anastomosis.">Current status and surgical technique for restorative proctocolectomy with ileal pouch-anal anastomosis.</a> Balkan Med J. 40 (4), 236-243 (2023).</li><li>Zhao, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbiota+DNA+translocation+into+mesentery+lymph+nodes+is+associated+with+early+development+of+pouchitis+after+ipaa+for+ulcerative+colitis.">Microbiota DNA translocation into mesentery lymph nodes is associated with early development of pouchitis after ipaa for ulcerative colitis.</a> Diseases of the Colon &amp; Rectum. 66 (11), e1107-e1118 (2022).</li><li>Ng, S. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Worldwide+incidence+and+prevalence+of+inflammatory+bowel+disease+in+the+21st+century:+A+systematic+review+of+population-based+studies.">Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies.</a> Lancet. 390 (10114), 2769-2778 (2017).</li><li>Pardi, D. S., D'haens, G., Shen, B., Campbell, S., Gionchetti, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+guidelines+for+the+management+of+pouchitis.">Clinical guidelines for the management of pouchitis.</a> Inflamm Bowel Dis. 15 (9), 1424-1431 (2009).</li><li>Shen, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+pouchitis,+Crohn's+disease,+cuffitis,+and+other+inflammatory+disorders+of+the+pouch:+Consensus+guidelines+from+the+international+ileal+pouch+consortium.">Treatment of pouchitis, Crohn's disease, cuffitis, and other inflammatory disorders of the pouch: Consensus guidelines from the international ileal pouch consortium.</a> Lancet Gastroenterol Hepatol. 7 (1), 69-95 (2022).</li><li>Dalal, R. L., Shen, B., Schwartz, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Management+of+pouchitis+and+other+common+complications+of+the+pouch.">Management of pouchitis and other common complications of the pouch.</a> Inflamm Bowel Dis. 24 (5), 989-996 (2018).</li><li>Li, K. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+rat+model+of+pouchitis+after+proctocolectomy+and+ileal+pouch-anal+anastomosis+using+2,4,6-trinitrobenzene+sulfonic+acid.">A new rat model of pouchitis after proctocolectomy and ileal pouch-anal anastomosis using 2,4,6-trinitrobenzene sulfonic acid.</a> J Gastrointest Surg. 25 (6), 1524-1533 (2021).</li><li>Drzymala-Czyz, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Discrepancy+between+clinical+and+histological+effects+of+dha+supplementation+in+a+rat+model+of+pouchitis.">Discrepancy between clinical and histological effects of dha supplementation in a rat model of pouchitis.</a> Folia Histochem Cytobiol. 50 (1), 125-129 (2012).</li><li>Santiago, P., Barnes, E. L., Raffals, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Classification+and+management+of+disorders+of+the+J+pouch.">Classification and management of disorders of the J pouch.</a> Am J Gastroenterol. 118 (11), 1931-1939 (2023).</li><li>Lichtman, S. N., Wang, J., Hummel, B., Lacey, S., Sartor, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+rat+model+of+ileal+pouch-rectal+anastomosis.">A rat model of ileal pouch-rectal anastomosis.</a> Inflamm Bowel Dis. 4 (3), 187-195 (1998).</li><li>Guarner, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inulin+and+oligofructose:+Impact+on+intestinal+diseases+and+disorders.">Inulin and oligofructose: Impact on intestinal diseases and disorders.</a> Br J Nutr. 93, S61-S65 (2005).</li><li>Kim, C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=L-tryptophan+exhibits+therapeutic+function+in+a+porcine+model+of+dextran+sodium+sulfate+(DSS)-induced+colitis.">L-tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis.</a> J Nutr Biochem. 21 (6), 468-475 (2010).</li><li>Valatas, V., Bamias, G., Kolios, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+colitis+models:+Insights+into+the+pathogenesis+of+inflammatory+bowel+disease+and+translational+issues.">Experimental colitis models: Insights into the pathogenesis of inflammatory bowel disease and translational issues.</a> Eur J Pharmacol. 759, 253-264 (2015).</li><li>Letson, H. L., Morris, J., Biros, E., Dobson, G. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conventional+and+specific-pathogen+free+rats+respond+differently+to+anesthesia+and+surgical+trauma.">Conventional and specific-pathogen free rats respond differently to anesthesia and surgical trauma.</a> Sci Rep. 9 (1), 9399 (2019).</li><li>Gu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Saccharomyces+boulardii,+a+yeast+probiotic,+inhibits+gut+motility+through+upregulating+intestinal+serotonin+transporter+and+modulating+gut+microbiota.">Saccharomyces boulardii, a yeast probiotic, inhibits gut motility through upregulating intestinal serotonin transporter and modulating gut microbiota.</a> Pharmacol Res. 181, 106291 (2022).</li><li>Akiyama, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endoscopic+phenotype+of+the+j+pouch+in+patients+with+inflammatory+bowel+disease:+A+new+classification+for+pouch+outcomes.">Endoscopic phenotype of the j pouch in patients with inflammatory bowel disease: A new classification for pouch outcomes.</a> Clin Gastroenterol Hepatol. 20 (2), 293-302 (2022).</li><li>Hata, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pouchitis+after+ileal+pouch-anal+anastomosis+in+ulcerative+colitis:+Diagnosis,+management,+risk+factors,+and+incidence.">Pouchitis after ileal pouch-anal anastomosis in ulcerative colitis: Diagnosis, management, risk factors, and incidence.</a> Dig Endosc. 29 (1), 26-34 (2017).</li><li>Gallo, G., Kotze, P. G., Spinelli, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgery+in+ulcerative+colitis:+When?+How.">Surgery in ulcerative colitis: When? How.</a> Best Pract Res Clin Gastroenterol. 32-33, 71-78 (2018).</li><li>Parks, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proctocolectomy+without+ileostomy+for+ulcerative+colitis.">Proctocolectomy without ileostomy for ulcerative colitis.</a> BMJ. 2, 85-88 (1978).</li><li>Shebani, K. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pouchitis+in+a+rat+model+of+ileal+J+pouch-anal+anastomosis.">Pouchitis in a rat model of ileal J pouch-anal anastomosis.</a> Inflamm Bowel Dis. 8 (1), 23-34 (2002).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Anhydrous ethanol</td>
			<td>Tianjin Fengchuan Chemical Reagent Technology Co., Ltd</td>
			<td>China</td>
			<td>Hematoxin-eosin Staining</td>
		</tr>
		<tr>
			<td>Dextran Sulfate Sodium&#160;</td>
			<td>&#160;Yeasen&#160;</td>
			<td>60316ES76</td>
			<td>Used to induce pouch inflammation</td>
		</tr>
		<tr>
			<td>Formaldehyde solution</td>
			<td>Tianjin Zhiyuan Reagent Company</td>
			<td>China</td>
			<td>Hematoxin-eosin Staining</td>
		</tr>
		<tr>
			<td>Gauze</td>
			<td>Jiangxi Zhonggan Medical Equipment Company</td>
			<td>China</td>
			<td>Used for animal microsurgery</td>
		</tr>
		<tr>
			<td>Hematoxylin</td>
			<td>Beijing Zhongshan Jinqiao Company</td>
			<td>China</td>
			<td>Hematoxin-eosin Staining</td>
		</tr>
		<tr>
			<td>Interferon &#947;&#160; Detection reagent kit</td>
			<td>Cloud-clone</td>
			<td>SEA049Ra</td>
			<td>Detecting inflammatory factors</td>
		</tr>
		<tr>
			<td>Interleukin-10 detection kit</td>
			<td>Cloud-clone</td>
			<td>SEA056Ra</td>
			<td>Detecting inflammatory factors</td>
		</tr>
		<tr>
			<td>Interleukin-17 detection kit</td>
			<td>Cloud-clone</td>
			<td>SEA063Ra</td>
			<td>Detecting inflammatory factors</td>
		</tr>
		<tr>
			<td>Interleukin-6 detection kit</td>
			<td>Cloud-clone</td>
			<td>SEA079Ra</td>
			<td>Detecting inflammatory factors</td>
		</tr>
		<tr>
			<td>Iodophor</td>
			<td>Tangpai Medical Equipment Co., Ltd</td>
			<td>China</td>
			<td>Used for animal microsurgery</td>
		</tr>
		<tr>
			<td>Microscopic manipulation instruments</td>
			<td>Aesculap</td>
			<td>Germany</td>
			<td>Used for animal microsurgery</td>
		</tr>
		<tr>
			<td>Occludin</td>
			<td>abcam</td>
			<td>ab216327</td>
			<td>Immunohistochemical testing</td>
		</tr>
		<tr>
			<td>Sewing needle</td>
			<td>Yangzhou Fuda Medical Equipment Co., Ltd</td>
			<td>China</td>
			<td>Used for animal microsurgery</td>
		</tr>
		<tr>
			<td>tumor necrosis factor &#945; Detection reagent kit</td>
			<td>Cloud-clone</td>
			<td>SEA133Ra</td>
			<td>Detecting inflammatory factors</td>
		</tr>
		<tr>
			<td>Two person binocular surgical microscope</td>
			<td>OPTON</td>
			<td>Germany</td>
			<td>Used for animal microsurgery</td>
		</tr>
		<tr>
			<td>Xylene</td>
			<td>Tianjin Yingda Rare and Precious Reagent Factory</td>
			<td>China</td>
			<td>Hematoxin-eosin Staining</td>
		</tr>
	</tbody>
</table>

a rat model of pouchitis following proctocolectomy and ileal pouch-anal anastomosis using dextran sulfate sodium

Pouchitis is a non-specific inflammation that affects the ileal pouch and is a prevalent complication following total proctocolectomy and ileal pouch-anal anastomosis (IPAA) in individuals with ulcerative colitis (UC)1,2,3. This condition has a relatively high occurrence rate of up to 50% and can cause various clinical manifestations, including diarrhea, abdominal pain, fecal blood loss, and fever. The exact cause of pouchitis remains elusive, although some researchers believe that a shift in the pouch flora may trigger immune activation and subsequent inflammation4,5,6,7.
Due to the challenges associated with conducting clinical trials on pouchitis, animal models can serve as valuable tools for studying pouchitis drugs and mechanisms. There are growing concerns regarding the creation of rat ileal pouches, with reports indicating possible inflammation8. However, research in this field remains sparse due to the intricate nature of the manufacturing process, which lacks clear guidelines9,10. In 1998, Lichtman was the first to establish an ileal pouch model in Lewis rats and Sprague-Dawley (SD) rats by performing total colectomy11. They observed macrophage infiltration, mucosal ulceration, and an increase in anaerobic bacterial flora within the intestines of these rats, providing a solid foundation for further research on ileal pouch inflammation. This experimental model of rat pouchitis closely mimics the physical signs and underlying mechanisms observed in human pouchitis.
Commonly applied preclinical ulcerative colitis models include the DSS and TNBS models. The inducing chemical 2,4,6-trinitrobenzene sulfonic acid (TNBS) typically simulates Crohn&#39;s disease12. The DSS model, respected for its efficacy, safety profile, and affordability, is often used as a reliable tool for UC induction due to the evident symptoms observed. Given the colonization of the pouch tissue, we successfully induced a pouchitis model using DSS13,14.
In the present study, microsurgery was used to successfully create an ileal pouch model in experimental rats via IPAA surgery. Subsequently, a sustained rat pouchitis model was established by inducing inflammation of the ileal pouch with DSS. Accuracy during surgery is essential for successful model formation, and postoperative care is crucial as well. This model can be used to investigate the pathogenesis of pouchitis, evaluate potential therapeutic agents, and further our understanding of this complex condition. The study streamlines the ileal pouch manufacturing procedure, reducing operation duration and boosting efficiency, thereby establishing a robust foundation for fundamental research into postsurgical pouch disorders.

<meta charset="utf8"></head><body>著者スポットライト:嚢炎の研究と治療のためのラットモデルの開発

デキストラン硫酸ナトリウムを用いた直腸結腸切除術および回腸嚢肛門吻合後の嚢炎のラットモデル

Ulcerative colitis (UC) is a chronic immune-mediated disease that affects the entire colon and rectum with a relapsing and remitting course, causing ...

a-rat-model-pouchitis-following-proctocolectomy-ileal-pouch-anal

Research

JoVE Journal

Medicine

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium

229 ビュー.  Tianjin Medical University General Hospital. 嚢炎の臨床試験の実施には課題があるため、動物モデルは嚢炎の薬とそのメカニズムを研究するための貴重なツールとして役立ちます。このプロトコルは、嚢炎のラットモデルを確立するための方法を説明しています。この嚢炎の実験動物モデルは、病状と治療を研究するための基礎を提供します。マウスの摂食とIPAA手術モデリングの経?...

ビデオ: 著者スポットライト:嚢炎の研究と治療のためのラットモデルの開発

Ulcerative colitis (UC) is a chronic immune-mediated disease that affects the entire colon and rectum with a relapsing and remitting course, causing lifelong morbidity. When medical treatment is ineffective, especially in cases of massive gastrointestinal bleeding, perforation, toxic megacolon, or carcinogenesis, surgery becomes the last line of defense to cure UC. Total colorectal resection and ileal pouch-anal anastomosis (IPAA) offer the best chance for long-term treatment. Pouchitis is the most common and troublesome postoperative complication. In this investigation, microsurgery is employed to create an ileal pouch model in experimental rats via IPAA surgery. Subsequently, a sustained rat model of pouchitis is established by inducing inflammation of the ileal pouch with dextran sulfate sodium (DSS). The successful establishment of rat pouchitis is validated through analysis of postoperative general status, weight, food and water intake, fecal data, as well as pouch tissue pathology, immunohistochemistry, and inflammatory factor analysis. This experimental animal model of pouchitis provides a foundation for studying the pathogenesis and treatment of the condition.

This protocol describes the method for establishing a rat model of pouchitis. The ileal pouch model was created by performing ileal pouch-anal anastomosis (IPAA) surgery using microsurgical techniques. After the surgery, the rat was treated with 4% dextran sulfate sodium (DSS) for 4 days.

Methods Article

著者スポットライト:嚢炎の研究と治療のためのラットモデルの開発