JoVE Journal

Biology

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Investigating Intestinal Inflammation in DSS-induced Model of IBD

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66.7K Views

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08:43 min

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February 1st, 2012

DOI :

February 1st, 2012

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Janice J. Kim¹, Md. Sharif Shajib¹, Marcus M. Manocha¹, Waliul I. Khan¹

¹Farncombe Family Digestive Health Research Institute, Department of Pathology and Molecular Medicine, McMaster University

Podsumowanie

Experimental models of inflammatory bowel disease have allowed us to examine the complex innate and adaptive immune responses associated with pathogenesis. Using histological scoring, quantification of pro-inflammatory cytokines and myeloperoxidase activity, one can begin to assess these responses seen in inflammatory bowel disease.

Rozdziały w tym wideo

0:05

Title

1:33

Collection of Colonic Tissue Samples and Assessment of Colitis Severity

3:58

Sample Preparation for Myeloperoxidase (MPO) Assay

5:09

Quantification of Inflammatory Markers

6:01

Evaluating the Severity of Acute Colitis in DSS-treated Animals

8:23

Conclusion

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