Measurement of Four Uterine NK Cell Subtypes Using Multiplexed Fluorescent Immunohistochemical Staining in Women with Repeated Implantation Failure

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08:29 min

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October 25th, 2024

DOI :

10.3791/67284-v

October 25th, 2024

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Lu Chen¹, Loucia Kit Ying Chan¹, Stacey Chui Yiu Wong¹, Joshua Jing Xi Li², Jacqueline Pui Wah Chung¹, Chi Chiu Wang¹^,³^,⁴^,⁵, Mingqing Li⁶^,⁷^,⁸, Tao Zhang¹

¹Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, ²Department of Pathology, School of Clinical Medicine, The University of Hong Kong, Queen Mary Hospital, ³Reproduction and Development Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, ⁴School of Biomedical Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, ⁵Chinese University of Hong Kong - Sichuan University Joint Laboratory in Reproductive Medicine, The Chinese University of Hong Kong, ⁶Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, ⁷NHC Key Lab of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Fudan University, ⁸Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University

Summary

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Uterine NK Cells

Multiplex Immunohistochemistry

Implantation Failure

Endometrial Receptivity

Phenotypes Identification

Reproductive Failure

Immune Status Assessment

Diagnostic Test

Immune Cell Subtypes

Early Pregnancy Processes

Decidualization

Biological Events Coordination

Chapters in this video

0:00

Introduction

1:35

Multiplex Immunohistochemistry for Uterine NK Cell Subtype Analysis in Endometrial Tissues

4:26

Image Acquisition After Multiplex Immuno‐Staining for Distinguishing uNK Cell Subtypes in Endometrial Tissues