Core - Analytical Chemistry

    Chapter 12

    Introduction to Molecular Spectroscopy

    12.1 : Dual Nature of Electromagnetic (EM) Radiation01:10

    12.1 : Dual Nature of Electromagnetic (EM) Radiation

    Electromagnetic (EM) radiation consists of electric and magnetic field components oscillating in planes perpendicular to each other and mutually ...

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    12.2 : Interaction of EM Radiation with Matter: Spectroscopy01:12

    12.2 : Interaction of EM Radiation with Matter: Spectroscopy

    Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in ...

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    12.3 : Molecular Spectroscopy: Absorption and Emission01:14

    12.3 : Molecular Spectroscopy: Absorption and Emission

    Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational ...

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    12.4 : Spectrophotometry: Introduction01:16

    12.4 : Spectrophotometry: Introduction

    Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a ...

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    12.5 : Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

    12.5 : Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

    Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and ...

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    12.6 : UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

    12.6 : UV–Vis Spectroscopy: Molecular Electronic Transitions

    In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of ...

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    12.7 : UV–Vis Spectrometers01:14

    12.7 : UV–Vis Spectrometers

    The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV ...

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    12.8 : UV–Vis Spectrum01:30

    12.8 : UV–Vis Spectrum

    When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs ...

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    12.9 : UV–Vis Spectroscopy: Beer–Lambert Law01:09

    12.9 : UV–Vis Spectroscopy: Beer–Lambert Law

    The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann ...

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    12.10 : Photoluminescence: Fluorescence and Phosphorescence01:23

    12.10 : Photoluminescence: Fluorescence and Phosphorescence

    Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs ...

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    12.11 : Variables Affecting Phosphorescence and Fluorescence01:26

    12.11 : Variables Affecting Phosphorescence and Fluorescence

    Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect ...

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    12.12 : Deactivation Processes: Jablonski Diagram01:25

    12.12 : Deactivation Processes: Jablonski Diagram

    Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The ...

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    12.13 : Photoluminescence: Applications01:14

    12.13 : Photoluminescence: Applications

    Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect ...

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    12.14 : Fluorescence and Phosphorescence: Instrumentation01:25

    12.14 : Fluorescence and Phosphorescence: Instrumentation

    Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select ...

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