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National Cancer Institute, NIH

4 ARTICLES PUBLISHED IN JoVE

Biology

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Katie L. DeCicco-Skinner¹, Gervaise H. Henry¹, Christophe Cataisson², Tracy Tabib¹, J. Curtis Gwilliam¹, Nicholas J. Watson¹, Erica M. Bullwinkle¹, Lauren Falkenburg¹, Rebecca C. O'Neill¹, Adam Morin¹, Jonathan S. Wiest²

¹Department of Biology, American University, ²Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

Cancer Research

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Raul Vizcardo^*1,2, S.M. Rafiqul Islam^*1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Meghan L. Good^1,2, Nicholas D. Klemen^1,2, Marta Bosch-Marce^1,2, Li Jia¹, Mikhael J. Kruhlak³, Nicholas P. Restifo^1,2

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

Cancer Research

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Meghan L. Good^*1,2, Raul Vizcardo^*1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Parisa Malekzadeh¹, Hiroshi Kawamoto³, Nicholas P. Restifo^1,2

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Immunology, Institute for Frontier Life and Medical Sciences, Kyoto University

This article describes a method to generate functional tumor antigen-specific induced pluripotent stem cell-derived CD8αβ⁺ single positive T cells using OP9/DLL1 co-culture system.

Medicine

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Luther M. Swift^1,2, Rafael Jaimes III^1,2, Damon McCullough^1,2, Morgan Burke^1,2, Marissa Reilly^1,2, Takuya Maeda^1,2,3, Hanyu Zhang⁴, Nobuyuki Ishibashi^1,2,3, Jack M. Rogers⁴, Nikki Gillum Posnack^1,2,5

¹Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Hospital, ²Children's National Heart Institute, Children's National Hospital, ³Center for Neuroscience Research, Children's National Hospital, ⁴Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, ⁵Department of Pediatrics, Department of Pharmacology & Physiology, School of Medicine and Health Sciences, George Washington University

The objective of this study was to establish a method for investigating cardiac dynamics using a translational animal model. The described experimental approach incorporates dual-emission optocardiography in conjunction with an electrophysiological study to assess electrical activity in an isolated, intact porcine heart model.