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Guangzhou University

9 ARTICLES PUBLISHED IN JoVE

Chemistry

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Juan Yao¹, Bruce W. Arey¹, Li Yang¹, Fei Zhang¹, Rachel Komorek¹, Jaehun Chun¹, Xiao-Ying Yu¹

¹Earth & Biological Sciences Directorate, Pacific Northwest National Laboratory

We present a procedure for real-time imaging and elemental composition analysis of boehmite particles in deionized water by in situ liquid Scanning Electron Microscopy.

Neuroscience

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Yangjian Feng^*1,2, Binliang Tang^*1,2, Ming Chen², Li Yang^1,3,4,5

¹School of Psychology, South China Normal University, ²School of Life Sciences, South China Normal University, ³Brain Science Institute, South China Normal University, ⁴Center for Studies of Psychological Application, South China Normal University, ⁵Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University

We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its success.

Genetics

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Ling Huang^*1, Xiaohui Deng^*1, Ronghua Li¹, Yanshi Xia¹, Guihua Bai², Kadambot H.M. Siddique³, Peiguo Guo¹

¹College of Life Sciences, Guangzhou University, ²Hard Winter Wheat Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, ³The UWA Institute of Agriculture, University of Western Australia

Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.

Neuroscience

Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

Shuiqing Chi¹, Mijing Fang¹, Kang Li¹, Li Yang², Shao-tao Tang¹

¹Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ²Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center

This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.

Immunology and Infection

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Xin Li¹, Zhengmei Mao², Li Yang¹, Kai Sun¹

¹Center for Metabolic and Degenerative Diseases, the Brown Foundation of Institute of Molecular Medicine, University of Texas Health Science Center at Houston, ²Microscopy Core, the Brown Foundation of Institute of Molecular Medicine, University of Texas Health Science Center at Houston

New blood vessel formation and sympathetic innervation play pivotal roles in adipose tissue remodeling. However, there remain technical issues in visualizing and quantitatively measuring adipose tissue. Here we present a protocol to successfully label and quantitatively compare the densities of blood vessels and nerve fibers in different adipose tissues.

Neuroscience

Evaluation of Hemisphere Lateralization with Bilateral Local Field Potential Recording in Secondary Motor Cortex of Mice

Yunan Chen^1,2, Ming Li³, Ying Zheng³, Li Yang¹

¹School of Life Sciences, Guangzhou University, ²Institute for Brain Research and Rehabilitation, South China Normal University, ³School of Life Sciences, South China Normal University

We present in vivo electrophysiological recording of the local field potential (LFP) in bilateral secondary motor cortex (M2) of mice, which can be applied to evaluate hemisphere lateralization. The study revealed altered levels of synchronization between the left and right M2 in APP/PS1 mice compared to WT controls.

Engineering

Finite Element Modeling for the Simulation of the Quasi-Static Compression of Corrugated Tapered Tubes

Xinmei Xiang^1,2, Dehua Shao¹, Xin Zhang³, Shaolin Zhang⁴, Yijie Liu¹

¹School of Civil Engineering, Guangzhou University, ²Guangdong Provincial Key Laboratory of Earthquake Engineering and Applied Technology, Guangzhou University, ³Department of Mechanics and Aerospace Engineering, Southern University of Science and Technology, ⁴School of Physics and Materials Science, Guangzhou University

This protocol describes the study of the quasi-static compression performance of corrugated tapered tubes using finite element simulations. The influence of the thickness gradient on the compression performance was investigated. The results show that proper thickness gradient design can change the deformation mode and significantly improve the energy absorption performance of the tubes.

Biology

Isolation and Whole-Cell Patch-Clamp Recording of Hippocampal Microglia from Adult Mice

Yu Chen¹, Cheng Long¹, Li Yang²

¹School of Life Sciences, South China Normal University, ²School of Life Sciences, Guangzhou University

The present protocol describes how to isolate and purify primary hippocampal microglia from adult mice, followed by instructions for conducting whole-cell patch-clamp recordings on these acutely isolated cells.

Biology

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

Jisheng Liu¹, Qiangjun Yang¹, Yang Yang¹, Xianfeng Lin¹

¹School of Life Sciences, Guangzhou University

Presented here is a protocol for chitosan/dsRNA nanoparticle delivery in silkworm Bombyx mori larvae to induce gene silencing through ingestion.