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Institute for Basic Science (IBS)

8 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Yang-Seok Park¹, Vijaya Sunkara¹, Yubin Kim¹, Won Seok Lee^1,2, Ja-Ryoung Han^1,3, Yoon-Kyoung Cho^1,4

¹Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, ²Agency for Defense Development (ADD), Daejeon, Republic of Korea, ³KOGAS (Korea Gas Corporation) Research Institute, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS)

This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.

Chemistry

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Byung Hyo Kim^1,2, Junyoung Heo^1,2, Won Chul Lee³, Jungwon Park^1,2

¹Center for Nanoparticle Research, Institute for Basic Science (IBS), ²School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, ³Department of Mechanical Engineering, Hanyang University

Here we introduce experimental protocols for the real-time observation of a self-assembly process using liquid-cell transmission electron microscopy.

Neuroscience

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Junhwan Kwon^1,2, Myunghwan Choi^1,2

¹Department of Biomedical Engineering, Sungkyunkwan University, ²Center for Neuroscience Imaging Research, Institute for Basic Science (IBS)

Here, we present a step-by-step protocol for imaging myelinated axons in a fixed brain slice using a label-free nanoscale imaging technique based on spectral reflectometry.

Neuroscience

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo

Jisoo Han^1,2, Pyonggang Choi³, Myunghwan Choi³

¹Department of Biomedical Engineering, Sungkyunkwan University, ²Center for Neuroscience Imaging Research, Institute for Basic Science, ³School of Biological Sciences, Seoul National University

The article introduces the µTongue (microfluidics-on-a-tongue) device for functional taste cell imaging in vivo by integrating microfluidics into an intravital imaging window on the tongue.

JoVE Journal

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Chi-Ju Kim^*1,2, Morgan D. Kuczler^*1, Liang Dong^1,3, Junyoung Kim^2,4, Sarah R. Amend¹, Yoon-Kyoung Cho^2,4, Kenneth J. Pienta¹

¹The Brady Urological Institute, Johns Hopkins University School of Medicine, ²Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), ³Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ⁴Center for Soft and Living Matter, Institute for Basic Science (IBS)

Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.

Biochemistry

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Yujin Kang¹, Subin Bae¹, Soyeong An¹, Ja Yil Lee^1,2

¹Department of Biological Sciences, Ulsan National Institute of Science and Technology, ²Center for Genomic Integrity, Institute for Basic Science (IBS)

DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.

Engineering

Fabrication of Micro-Patterned Chip with Controlled Thickness for High-Throughput Cryogenic Electron Microscopy

Min-Ho Kang^1,2, Minyoung Lee^3,4, Sungsu Kang^3,4, Jungwon Park^3,4,5,6

¹Department of Biomedical-Chemical Engineering, The Catholic University of Korea, ²Department of Biotechnology, The Catholic University of Korea, ³School of Chemical and Biological Engineering, and Institute of Chemical Processes, Seoul National University, ⁴Center for Nanoparticle Research, Institute of Basic Science (IBS), ⁵Institute of Engineering Research, College of Engineering, Seoul National University, ⁶Advanced Institutes of Convergence Technology, Seoul National University

A newly developed micro-patterned chip with graphene oxide windows is fabricated by applying microelectromechanical system techniques, enabling efficient and high-throughput cryogenic electron microscopy imaging of various biomolecules and nanomaterials.

Behavior

Observational Fear as a Model of Affective Empathy in Mice

Hyoin Lee¹, Dahm So^1,2, Jiye Choi¹, Seungmoon Jung¹, Sehoon Keum¹

¹Center for Cognition and Sociality, Institute for Basic Science (IBS), ²Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST)

The observational fear paradigm assesses vicarious freezing in rodents as a model for affective empathy. This procedure entails exposing observer rodents to conspecific demonstrators receiving aversive foot shocks to elicit empathic freezing responses. By employing observational fear assays, researchers can investigate the neural mechanisms underlying affective empathy.