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Hampton University

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Preparation and Characterization of Lipophilic Doxorubicin Pro-drug Micelles

Feng Li¹, Candace Snow-Davis¹, Chengan Du¹, Mikhail L. Bondarev¹, Marilyn D. Saulsbury¹, Simone O. Heyliger¹

A protocol for the preparation and characterization of lipophilic doxorubicin pro-drug loaded 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) micelles is described.

Medicine

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

Zhongwei Sun^1,2, Feng Li³, Xin Zhou³, Wen Wang^1,2

¹Cellular Biomedicine Group, Shanghai, ²Cellular Biomedicine Group, Cupertino, ³Department of Respiratory Medicine, Shanghai General Hospital, Shanghai Jiaotong University

This study describes the successful generation of a new chronic obstructive pulmonary disease (COPD) animal model by repeatedly exposing mice to high concentrations of ozone.

Neuroscience

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method

Weiyue Zhang^*1, Wei Zhang^*1, Ning Dai^*1, Chenxia Han², Fengzhi Wu¹, Xu Wang¹, Libo Tan¹, Jie Li¹, Feng Li¹, Qingjia Ren³

¹School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, ²Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, ³Institute of Tibetan Medicine, Tibetan Traditional Medical College

Here, we present a protocol to introduce a rat model of central fatigue using the modified multiple platform method (MMPM).

JoVE Journal

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Dan Li^*1, Feng Li^*1, Pavithran Guttipatti¹, Yuanquan Song^1,2

¹Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine, University of Pennsylvania

Here, we present a protocol using the Drosophila sensory neuron - dendritic arborization (da) neuron injury model, which combines in vivo live imaging, two-photon laser axotomy/dendriotomy, and the powerful fly genetic toolbox, as a platform for screening potential promoters and inhibitors of neuroregeneration.

Environment

Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri (Uljanin 1884) for Experimental Studies

Tina L. Walters¹, Deidre M. Gibson², Marc E. Frischer¹

¹Skidaway Institute of Oceanography, University of Georgia, ²Hampton University

Doliolids, including the species Dolioletta gegenbauri, are small gelatinous marine zooplankton of ecological significance found on productive subcontinental shelf systems worldwide. The difficulty of culturing these delicate organisms limits their investigation. In this study, we describe cultivation approaches for collecting, rearing, and maintaining the doliolid Dolioletta gegenbauri.