The Babraham Institute
3 ARTICLES PUBLISHED IN JoVE
Biology
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Eleftherios Karanasios 1, Eloise Stapleton 2, Simon A. Walker 1, Maria Manifava 1, Nicholas T. Ktistakis 1
1Signalling Programme, The Babraham Institute, 2MRC Group, Cardiff School of Biosciences, Cardiff University
Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.
Biology
Robust 3D DNA FISH Using Directly Labeled Probes
Daniel J. Bolland 1, Michelle R. King 2, Wolf Reik 2,3, Anne E. Corcoran 1, Christel Krueger 2
1Nuclear Dynamics Programme, The Babraham Institute, 2Epigenetics Programme, The Babraham Institute, 3Centre for Trophoblast Research, University of Cambridge
We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.
Biology
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
Felipe J. Renna 1, Malena Herrera Lopez 1, Maria Manifava 2, Nicholas T. Ktistakis 2, Maria I. Vaccaro 1
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute
The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.
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