Diptheria Toxin (DPT) Myocarditis Induction and Light-sheet Microscope Sample Preparation
2:12
Light-sheet Microscopy and Image Post-processing
5:16
Results: Representative 3D Recordings of Whole Organ Light-sheet Microscopy
5:58
Conclusion
Transcrição
The overall goal of this protocol is to chemically clear the heart of a mouse, with an induced cardiac arrhythmia for a whole organ visualization of the leukocytic cardiac infiltrate by Fluorescence Light Sheet Microscopy. This method can help ans
Faça login ou inicie seu teste gratuito para acessar este conteúdo
Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.