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Jiangsu Avatarget Biotechnology Co., Ltd.

4 ARTICLES PUBLISHED IN JoVE

Environment

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Zhuo Li^1,2, Fangting Ai³, Jing Zhang⁴, Zhenyang Yu^1,2, Daqiang Yin^1,2

¹College of Environmental Sciences and Engineering, Tongji University, ²Shanghai Institute of Pollution Control and Ecological Security, ³Jiaxing Tongji Institute of Environment, ⁴College of Ecological Technique and Engineering, Shanghai Institute of Technology

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

Engineering

Measurement of Chladni Mode Shapes with an Optical Lever Method

Rong Feng^*1, Yan Luo^*1, Yixue Dong¹, Mengke Ma², Yuqi Wang², Jing Zhang³, Wenjiang Ma³, Donghuan Liu⁴

¹School of Materials Science and Engineering, University of Science and Technology Beijing, ²School of Civil and Resource Engineering, University of Science and Technology Beijing, ³Basic Experimental Center for Natural Science, University of Science and Technology Beijing, ⁴School of Mathematics and Physics, University of Science and Technology Beijing

A simple method of measuring the Chladni mode shape on an elastic plate by the principle of an optical lever is proposed.

Cancer Research

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Jing Zhang¹, Midory Thorikay¹, Gerard van der Zon¹, Maarten van Dinther¹, Peter ten Dijke¹

¹Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.

Cancer Research

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Wei Hou^*1,2, Zilin Zhang^*3,4, Jing Zhang^3,4, Shihui Xu^2,4, Menglan Li⁴, Xiaoran Li^2,4, Zaozao Chen^2,3, Cailian Wang^1,2

¹Department of Oncology, School of Medicine, Southeast University, ²State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, ³Institute of Medical Devices (Suzhou), Southeast University, ⁴Jiangsu Avatarget Biotechnology Co., Ltd.

This article demonstrates a standardized method for constructing three-dimensional tumor spheroids. A strategy for spheroid observation and image-based deep-learning analysis using an automated imaging system is also described.