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South China Agricultural University

9 ARTICLES PUBLISHED IN JoVE

Isolation and In Vitro Decidualization of Mouse Primary Endometrial Stromal Cells

Yuefang Liu¹, Zengming Yang¹, Xiaohuan Liang¹

¹College of Veterinary Medicine, South China Agricultural University

We aim to provide a method for the isolation and culturing of mouse endometrial stromal cells by enzymatic digestion. The isolated stromal cells are of high purity and can be decidualized by hormones in vitro. This protocol represents a feasible and stable method for stromal cell culture and for the further study of decidualization.

Chemistry

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Xiaomin Peng^*1, Guitao Zhong^*1, Hao Wang¹

¹College of Life Sciences, South China Agricultural University

We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. It takes advantage of using a single expression plasmid that contains multiple functionally independent protein expressing cassettes to achieve protein co-expression.

Environment

Choice and No-Choice Bioassays to Study the Pupation Preference and Emergence Success of Ectropis grisescens

Cai Wang¹, Huifang Wang¹, Tao Ma^1,2, Qiang Xiao³, Panrong Cao⁴, Xuan Chen⁵, Hongpeng Xiong¹, Wenquan Qin¹, Zhaohui Sun¹, Xiujun Wen¹

¹Guangdong Key Laboratory for Innovation Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, ²College of Agriculture, South China Agricultural University, ³Tea Research Institute, Chinese Academy of Agricultural Sciences, ⁴College of Horticulture, South China Agricultural University, ⁵College of Coast and Environment, Louisiana State University

Here, we present a protocol to investigate the pupation preference of mature larvae of Ectropis grisescens in response to soil factors (e.g., substrate type and moisture content) using choice bioassays. We also present a protocol of no-choice bioassays to determine the factors that affect the pupation behaviors and survivorship of E. grisescens.

Neuroscience

Investigating Alterations in Caecum Microbiota After Traumatic Brain Injury in Mice

Liang Wen^*1, Wendong You^*1, Yadong Wang^*1, Yuanrun Zhu¹, Hao Wang¹, Xiaofeng Yang¹

¹Emergency and Trauma Center, The International Medical Center, The First Affiliated Hospital, School of Medicine, Zhejiang University

Presented here is a protocol to induce diffuse traumatic brain injury using a lateral fluid percussion device followed by the collection of the caecum content for gut microbiome analysis.

Genetics

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Yafeng Zhang^1,2, Xun Liao², Peihua Luo², Kui Peng², Wenyi Ye², Tengxiang Lian^1,2, Qibin Ma^1,2, Hai Nian^1,2

¹State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, ²Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University

We present a circular RT-PCR-based strategy by combining circular RT-PCR, quantitative RT-PCR, RNA 5' polyphosphatase-treatment, and Northern blot. This protocol includes a normalization step to minimize the influence of unstable 5' triphosphate, and it is suitable for discriminating and mapping the primary and processed transcripts stably accumulated in maize mitochondrion.

Medicine

Application of Straight-needle, Three-tailed, Knot-free, Peritoneal Sutures in Laparoscopic Transabdominal Preperitoneal Hernia Repair

Ning Ma^*1, Zhen Zong^*2, Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Jia-lin Chen¹, Shuang Chen¹, Tai-cheng Zhou¹

¹Department of Gastroenterological Surgery and Hernia Center, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, ²Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Nanchang University

Here, we present a protocol to introduce a new peritoneal suture method. This method is called straight-needle, three-tailed, knot-free suture, and we will outline the manufacturing method and clinical application of this suture in detail.

Medicine

A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs

Xiaohui Li^*1,2, Weijiang Tan^*1,3, Xiang Li^*1, Shuang Zheng^*1, Xiaoshen Zhang², Honghua Chen¹, Zhanhong Pan¹, Caiyi Zhu¹, Feng Hua Yang¹

¹Guangdong Laboratory Animals Monitoring Institute, ²Department of Cardiovascular Surgery, the First Affiliated Hospital, Jinan University, ³College of Veterinary Medicine, South China Agricultural University

The present protocol describes a step-by-step procedure to establish a minipig model of heart failure with preserved ejection fraction using descending aortic constriction. The methods for evaluating cardiac morphology, histology, and function of this disease model are also presented.

Medicine

Establishment and Evaluation of a Porcine Vein Graft Disease Model

Xiaohui Li^*1, Jinlin Hu^*1, Weijiang Tan^*2, Zhaoming Lin^*1, Caiyi Zhu², Chengfeng Huang¹, Jiawen Huang³, Yintian Liu¹, Qiuying Liao¹, Hua Lu¹, Xiaoshen Zhang¹

¹Department of Cardiovascular Surgery, the First Affiliated Hospital of Jinan University, ²Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, ³The First Affiliated Hospital of Jinan University

In this protocol, novel pig vein bypass grafting was performed through a small incision in the left chest wall without cardiopulmonary bypass. A postoperative pathology study was done, which showed intimal thickening.

Medicine

Application of a New Mesh Fixation Method in Laparoscopic Incisional Hernia Repair

Ning Ma^*1, Dai Tang^*2, Fu-xing Tang¹, En-min Huang¹, Tao Ma¹, Wei-sheng Yang¹, Chuang-xiong Liu¹, Hao-nan Huang¹, Shuang Chen¹, Tai-cheng Zhou¹

¹Department of Gastroenterological Surgery and Hernia Center, The Sixth Affiliated Hospital, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhou, Sun Yat-sen University, ²Department of Gastrointestinal Surgery, the Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi)

Presented here is a method for improving the mesh placement in laparoscopic incisional hernia repair, which can shorten the time required for mesh fixation and reduce the occurrence of postoperative chronic pain.