JoVE Logo
Centro de Recursos para Docentes

Entrar

Measuring Exocytosis in Neurons Using FM Labeling

DOI :

10.3791/117-v

November 30th, 2006

November 30th, 2006

15,699 Views

1Department of Molecular and Cell Biology, Harvard

The ability to measure the kinetics of vesicle release can help provide insight into some of the basics of neurotransmission. Here we used real-time imaging of vesicles labeled with the red fluorescent dye FM 4-64 to measure the rate of presynaptic vesicle release in hippocampal neuronal cultures.

Tags

Measuring

-- Visualizações

Vídeos Relacionados

article

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro

article

Calcium Imaging of Cortical Neurons using Fura-2 AM

article

Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method

article

Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

article

Measuring Fast Calcium Fluxes in Cardiomyocytes

article

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

article

Measuring Respiratory Function in Mice Using Unrestrained Whole-body Plethysmography

article

Measuring Glutathione-induced Feeding Response in Hydra

article

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

article

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

JoVE Logo

Privacidade

Termos de uso

Políticas

Pesquisa

Educação

SOBRE A JoVE

Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados