Setting Up the Perfusion Chamber to Measure Contractility in Isolated, Pressurized Lymph Vessels

To begin, take borosilicate glass micro-pipettes acquired from a commercial source. Cut and polish the micro-pipettes to a length of approximately one to two centimeters for use in the isolated vessel perfusion chamber. Connect each mounted glass cannula in the vessel perfusion chamber to independent pressure transducers aligned with independent gravity-fed pressure regulators and connected using polyethylene tubing.

Backfill the profusion chamber, glass micro-pipettes, and the gravity-fed pressure regulator along with the polyethylene tubing with the physiological salt solution, then clamp the pressure to ensure the cannulas are not pressurized.