Preparation of the Chitosan/dsRNA Nanoparticles for Feeding-Based RNA Interference in Silkworms

To begin mix 0.1 molar sodium acetate and 0.1 molar acetic acid in deionized water at pH 4.5. Then prepare 100 millimolar sodium sulfate buffer in deionized water at room temperature. Weigh the commercialized chitosan to prepare a 0.02%weight by v