Coating Culture Plates with Extra-cellular Matrix Proteins
2:15
Isolation of Fetal Rodent Lung Type II Epithelial Cells
5:32
Isolation of Fetal Rodent Lung Fibroblasts
6:15
Experimental System to Provided Mechanical Stimulation to Lung Cells
7:42
Representative Results from Mechanotransduction Experiments Using Fetal Lung Cells
9:15
Conclusion
Mechanical forces play a key role in lung development and lung injury. Here, we describe a method to isolate rodent fetal lung type II epithelial cells and fibroblasts and to expose them to mechanical stimulation using an in vitro system.