September 17th, 2012
•Among the three mosquito genera, namely Anopheles, Aedes, and Culex, physical genome mapping techniques were established only for Anopheles, whose members possess readable polytene chromosomes. For the genera of Aedes and Culex, however, cytogenetic mapping remains challenging because of the poor quality of polytene chromosomes. Here we present a universal protocol for obtaining high-quality preparations of mitotic chromosomes and an optimized FISH protocol for all three genera of mosquitoes.
Tags
Vídeos Relacionados
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
2D and 3D Chromosome Painting in Malaria Mosquitoes
Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
Vector Competence Analyses on Aedes aegypti Mosquitoes using Zika Virus
Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados