April 19th, 2013
•The combination of chromatin immunoprecipitation and ultra-high-throughput sequencing (ChIP-seq) can identify and map protein-DNA interactions in a given tissue or cell line. Outlined is how to generate a high quality ChIP template for subsequent sequencing, using experience with the transcription factor TCF7L2 as an example.
Tags
Vídeos Relacionados
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
Targeted DNA Methylation Analysis by Next-generation Sequencing
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Modified Methods for Loading of High-Throughput DNA Extraction Plates Reduce Potential for Contamination
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados