August 13th, 2020
•This paper describes a semiautomated, microscaled ChIP-Seq protocol of DNA regions associated with a specific histone modification (H3K27ac) using a ChIP liquid-handler platform, followed by library preparation using tagmentation. The procedure includes control assessment for quality and quantity purposes and can be adopted to other histone modifications or transcription factors.
Vídeos Relacionados
Large-Scale Screens of Metagenomic Libraries
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Large Scale Zebrafish-Based In vivo Small Molecule Screen
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Infinium Assay for Large-scale SNP Genotyping Applications
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados