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JoVE Journal

Bioengineering

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Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study

16.3K Views

20:24 min

January 31st, 2014

January 31st, 2014

16,349 Views

0:05

Title

1:13

Planning a DoE Strategy

3:15

Plant Cultivation

4:12

Transient Protein Expression

7:02

Protein Quantitation

8:35

Data Analysis and Evaluation

11:53

Results: The DoE Strategy Examines Major Factors and Incubation Conditions that Impact Transient Expression of Proteins in Plants

20:01

Conclusion

We describe a design of experiments approach that can be used to determine and model the influence of transgene regulatory elements, plant growth and development parameters, and incubation conditions on the transient expression of monoclonal antibodies and reporter proteins in plants.

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