November 14th, 2014
•The molecular mechanisms that co-ordinate the formation of inhibitory GABAergic synapses during ontogeny are largely unknown. To study these processes,we have developed a co-culture model system which incorporates embryonic medium spiny GABAergic neurons cultured together with stably transfected human embryonic kidney 293 (HEK293) cells expressing functional GABAA receptors.
Vídeos Relacionados
Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells
Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados