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Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

DOI :

10.3791/52749-v

April 29th, 2015

April 29th, 2015

35,303 Views

1Centre de recherche du Centre hospitalier de l'Université de Montréal, Institut du cancer de Montréal, 2Département de Médecine, Université de Montréal

We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.

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