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O isolamento de regiões específicas genômico e identificação de moléculas pela Associated enChIP

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09:26 min

January 20th, 2016

January 20th, 2016


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Title

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Preparation of Tagged Chromatin

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Chromatin Immunoprecipitation

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Immunoprecipitated Chromatin Analysis

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Results: enChIP to Telomere and the Interferon Regulatory Factor-1 (IRF-1) Promoter Regions

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Conclusion

Transcrição

The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the functions of these regions. This protocol describes procedures to perform engineered DNA-binding molecule-mediated chromatin imunoprecipitation (enChIP) for identification of proteins and RNAs associated with a specific genomic region.

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