The overall goal of this procedure is to use a Genetically Encoded FRET Bile Acid Sensor to easily visualize bile acid transport and dynamics with high spacial and temporal resolution. This sensor can answer key metabolic questions, such as the re
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We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.