The overall goals of the outgrowth culture method and the ex vivo migration assay are to isolate primary cultures of epicardial cells for studying epithelial to mesenchymal transitions and to assess the functional migration of epicardial derived c
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Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.