Seeding Human Embryonic Stem Cells onto the Stenciled Substrate
4:39
Stencil Removal and Passivation of Unpatterned Substrate
6:20
Results: Mechanically Patterning Early Mesoendermal Differentiation
7:39
Conclusion
Transcrição
The overall goal of this stencil-based micropatterning method, for human pluripotent stem cells, is to provide a simple and robust means to generate spatial environmental gradients that control stem cell differentiation patterns. This method can h
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Human pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns; although this requires the presentation of spatial environmental gradients. We present stencil micropatterning as a simple and robust method to generate biochemical and mechanical gradients for controlling hPSC differentiation patterns.