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JoVE Journal

Bioengineering

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Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

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10:14 min

November 18th, 2016

November 18th, 2016

6,281 Views

0:05

Title

0:58

Modifying a Polystyrene Surface for Printing

2:29

Etching Patterns into the Polystyrene Sheet

5:48

Preparing and Printing Cell-containing Gelatin Bioink into the Etched Patterns

8:50

Results: Constraint of Printed Stem Cells

9:29

Conclusion

Transcrição

The overall goal of this automated robotic dispensing technique is to create a surface for topographical cell guidance and then deliver cells to these features following a programmed pattern, allowing control over cell behavior and distribution. T

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This protocol describes a bioprinting methodology using an automated robotic depositing system that incorporates etched topographical guidance cues with the precision deposition of a cell bearing hydrogel bioink. The printed cells are directly delivered to the etched features and are able to sense and orientate with them.

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