Results: PDX-dissociated Breast Cancer Cell Transduction Using High Titer Lentiviral Vectors
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Conclusion
Transcrição
The overall goal of this transduction protocol is to stably introduce reporter genes in patient-derived xenografts, or PDXs, to facilitate their use in experimental and spontaneous metastasis models. This method can help answer key questions in ca
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We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.