Oxygen-Glucose Deprivation (OGD) of Primary Astrocytes
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Representative Results
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Conclusion
TRANSCRIÇÃO
The following protocol is intended to show the isolation of a primary astrocytes from brains of pup rats to perform oxygen glucose deprivation or OGD. Exposure of purified astrocytes, to oxygen glucose deprived environment is a module that allows
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Ischemic stroke is a complex event in which the specific contribution of astrocytes to the affected brain region exposed to oxygen glucose deprivation (OGD) is difficult to study. This article introduces a methodology to obtain isolated astrocytes and study their reactivity and proliferation under OGD conditions.