Results: Murine Astrocyte Cultures to Study Direct Neuronal Reprogramming
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Conclusion
Transcrição
Astrocytes are an interesting autogenous population to target for direct neuronal programming. This protocol provides a reliable technique to isolating culture astrocytes with high purity from different region or the central nervous system. This p
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Here we describe a detailed protocol to generate highly enriched cultures of astrocytes derived from different regions of the central nervous system of postnatal mice and their direct conversion into functional neurons by the forced expression of transcription factors.